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| Drosophila Embryo Injection Services | |||
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Drosophila Embryo Injection ServicesWe provide high quality Drosophila transgenic service to both research institutions and companies. We offer you partial to full service ranging from DNA preparation, embryo microinjection, screening for white+, yellow+, vermilion+ and/or Fluorescence (GFP/RFP/DsRed) phenotypes, to balancing crosses. Most importantly, the costs of our Drosophila injection services are more reasonable and affordable compared to that of others. With a large number of facilities and the highly experienced staff, we are able to initiate the process immediately upon receiving your sample. Our friendly web-based database allows you to track your up-to-the-dated fly injection status, service history and more. See here for the sample page. P-element TransformationThe Drosophila P-element transformation usually involves the injection of transposon-based construct with the white and/or the yellow marker to w1118 or yw host line. We perform injections with your (or our) purified plasmid construct (helper P-element plasmid will be supplied by us unless you prefer to use your own) and return you the injected lavae, or the stable lines or balanced lines depending on your chosen service. For details, please refer to the service page. PhiC31 integrase-mediated transgenesis systems - P[acman] and FlyC31PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site (attB) and a phage attachment site (attP). We will inject your purified attB construct into your selected PhiC31-containing attP docking site embryos and return you the injected larvae, the stable lines without the PhiC31 source or the balanced lines (for the FlyC31 system, you may also choose to remove most of the non-necessary sequences by the Cre-mediated excision, leaving ONLY the gene-of-interest flanking with one loxP and one attL site) depending on your chosen service. For details, please refer to the service page and the PhiC31 information page.Molecular Biology ServicesWe also provide related molecular lab services, e.g. DNA preparation (Qiagen Midiprep), BAC preparation (Qiagen Large Construct Maxiprep), PCR confirmation of recombination sites, orientation screening for MiMIC lines, etc. For details, please refer to the service page. |
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