BestGene Inc.

thebestgene.com (Bestgene Inc) provides fast, cheap and high quality Drosophila transgenic (transgene/injection/microinjection/p-element transformation) service. (Drosophila, fly, fruit fly, P-element, embryo, Transgenic, microinjectoin, injection, transformation, transgene, service, facility, research, transgene service, Drosophila transgene, fly transgene, fruit fly transgene, Drosophila injection, fly injection, fruit fly injection, Drosophila microinjection, fly microinjection, fruit fly microinjection, p-element transformation service, Drosophila embryo microinjection, embryo microinjection, transposon injection, P-element injection, transgenic fruitfly, transgenic fly, transgenic Drosophila)

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	Drosophila Embryo Injection Services

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User friendly system! Real-time tracking of service progress online
Inject 200+/300+ embryos per service
Selection of w¹¹¹⁸, yw or your own stock
Screen for w⁺, y⁺, v⁺ and/or GFP/RFP/DsRed/YFP/CFP
Deliver transformed and/or balanced flies. We do the crosses!
One shipping & handling charge per order! No matter how many services purchased
PhiC31 integrase-mediated site-specific transgenesis
MiMIC injection service
CRISPR injection service
Over 120,000 individual constructs were successfully injected and over 630,000 transformants delivered!

Drosophila Embryo Injection Services

We provide high quality Drosophila transgenic service to both research institutions and companies. We offer you partial to full service ranging from DNA preparation, embryo microinjection, screening for white⁺, yellow⁺, vermilion⁺ and/or Fluorescence (GFP/RFP/DsRed/YFP/CFP) phenotypes, to balancing crosses. With a large number of facilities and our experienced staffs, we are able to handle library-scale injection projects. Our friendly web-based database allows you to track your fly injection status, service history and more. See here for the sample page.

P-element Transformation

The Drosophila P-element transformation usually involves the injection of transposon-based construct with the white and/or the yellow marker to w¹¹¹⁸ or yw. We perform injections with your (or our) purified plasmid construct (helper P-element plasmid will be supplied by us unless you prefer to use your own) and return you the stable lines or the balanced lines depending on your chosen service. For details, please refer to the service page.

PhiC31 integrase-mediated transgenesis systems - P[acman] and FlyC31

PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site (attB) and a phage attachment site (attP). We will inject your purified attB construct into your preferred PhiC31-containing attP docking site embryos and return you the stable lines without the PhiC31 source or the balanced lines depending on your chosen service. For details, please refer to the service page and the PhiC31 information page.

CRISPR Genome Editing

The Drosophila CRISPR/Cas9 system are a powerful genome engineering tool that are composed of programmable, sequence-specific DNA-binding modules associated with a nonspecific DNA nuclease, which induce DSB (DNA double-strand breaks) that stimulate NHEJ (non-homologous end joining) or HR (homologous recombination) repairing mechanisms at specific genomic locations. For details, please refer to the service page and the Genome Editing page.

Molecular Biology Services

We also provide related molecular lab services, e.g. DNA preparation (Qiagen Midiprep), BAC preparation (Qiagen Large Construct Maxiprep), transposable element mapping for P-element lines, molecular mapping for CRISPR HDR lines, PCR confirmation of recombination sites, orientation screening for MiMIC lines, etc. For details, please refer to the service page.

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